CLSM - Leica SP8 inverse (Balgrist Campus)

SP8inverse_Balgrist

The Leica SP8 is an automated inverse confocal laser scanning microscope allowing simultaneous aquisition of 3 fluorescent channels and 1 transmitted light channel (BF or DIC).

Location

University Zurich, Balgrist Campus, Lengghalde 5, 8008 Zurich, Room Microscopy W.112, Phone +41 44 510 7338

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Light sources and lasers

  • Halogen lamp for transmitted light
  • External fluorescence lamp  LQ-HXP 120
  • Solid state diode lasers: 405 nm (50 mW), 488 nm (20 mW), 552 nm (20 mW) and  638 nm (30 mW)

Scanners

Dual scanning system:

Regular scanner: 10 - 1800 Hz

Resonant scanner: 8000 Hz

Objectives

Name Magnification NA Immersion WD (mm)
HC PL APO 10x 0.3 Air 11
HC PL APO CS2 20x 0.75 Imm 0.67
HCX PL APO 37°C CS2 63x 1.3 Glycerol 0.3

Detector System

Two Hybrid (HyD) detectors, one photomultiplier tube (PMT) detector and one additional PMT for transmission. Excitation controlled by AOTF, beam splitters: RT 15/85, substrate, TD 488/552/638, DD 488/552 for separation between excitation and emission. Accurate emission detection by spectral detectors.

Fluorescence Filters for widefield

Name Excitation Range Excitation Filter Dichroic Emission Filter
A4 UV BP 360/40 400 BP 470/40
GFP blue BP 470/40 500 BP 525/50
N2.1 green BP 515-560 580 LP 590
CFP violet/blue BP 436/20 455 BP 480/40
I3 blue BP 450/490 410 LP 515

Available Accessories

  • controlled environmental conditions (CO2, temperature, humidity)
  • Matrix screening software (reservation needed)

 

 

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

 

 

Remarks

This microscope acquires images with improved axial resolution compared with more coventional widefield microscopes. In addition to the multicolor images the microscope can acquire large tile scans fully automatically. The microscope allows live imaging with controlled environmental conditions (CO2, temperature, humidity).

Literature and Links

Further information (internal UZH use only)

Follow this link for further background information, documents and links

Responsible Persons

If you have questions about the microscope please contact Caroline Aemisegger or Jana Döhner.