The mesoSPIM (mesoscale selective plane illumination microscopy) is the ideal instrument to quickly bridge scales from the µm- to the cm-level, which enables it to serve as an excellent tool for detailed three-dimensional anatomical investigations in many biological and medical fields (Voigt et al., 2019).
- Compatibility with all major clearing techniques Clarity and iDISCO.
- Large field-of-views, ranging from 2 to 20 mm using an Olympus MVX-10 macroscope.
- Dual-sided excitation. Optimized for fast screening of whole mouse brain samples, yielding relatively small datasets (10-14 GB/brain/color) near-isotropic sampling (5-10 µm in X,Y & Z) due to an axially swept lightsheet which leads to uniform z-resolution across the field-of-view.
- Modular sample holders and quick sample exchange.
- Large travel range (45 x 45 x 100 mm) to allow imaging of very large samples.
For further information: MesoSPIM.org
The mesoSPIM excitation path is based on axially scanned light-sheet microscopy (ASLM). The waist of the light-sheet is translated through the sample in synchrony with the rolling shutter of the camera. The waist is translated with electrically tunable lenses by Optotune.
Light Sources and Lasers
The iChrome MLE comprises 3 diode lasers and one DPSS laser fully integrated in one compact housing. Lasers: 405nm (100mW), 448nm (100mW), 561nm (100mW), 640nm (100mW)
The mesoSPIM detection path consists of an Olympus MVX-10 macroscope with a MVPLAPO 1x objective, which allows FOVs of 2-20 mm in combination with a Hamamatsu Orca Flash 4.0 V3 camera.
|Quadband filter 405/488/561/640||BP444/27; BP523/22; BP594/20; BP704/46|
|515 LP||LP 515|
|561 LP||LP 568|
A Digital sCMOs-Camera: Pixel Array 2048 x 2048 (pixel size 6.5 µM), (Hamamatsu ORCA-FLASH 4.0 V3).
The microscope software, mesoSPIM-control, is written in Python and allows users to specify sequences of z-stacks using a table-based acquisition manager. The software can also be used to acquire large-scale tiling acquisitions.
- For initial tests and the first experiments, the ZMB will serve users with the needed experimental accessories such as cuvettes and immersion solutions. If they become regular user of the microscope, they have to organize the accessories by themselves.