Multiphoton - Leica SP8 MP DIVE FALCON (Irchel)

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The upright Leica multiphoton and confocal fluorescence microscope is equipped with a pre-compensated infrared laser (Insight DS+ Dual from Spectra Physics) for non linear fluorescence excitation and second harmonics generation as well as with a white light laser for single photon excitation.

The 4 channel tuneable non-descanned DIVE detection unit allows for unprecedented spectral flexibility in multiphoton experiments. 

Six FLIM enabled HyD detectors together with the fast FALCON FLIM implementation allow for highly efficient multidimensional FLIM imaging in confocal as well as multiphoton operation.

Location

University Zurich, Irchel Campus, Room Y42-H-77. Pone: +41 44 635 53 44.

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Light Sources and Lasers

  • Insigth DS+ Dual (680-1300 nm & 1041nm) ultrafast NIR laser for multiphoton excitation
  • White light laser (WLL): continuous laser output in the range of 470-670nm; AOTF allows to choose up to 8 different wavelengths simultaneously; pulsed laser system with a flexible repetition rate up to 80 MHz (Pulse picker)
  • Diode laser 405 nm (50mW)
  • Argon Laser (65mW): 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
  • Halogen lamp for transmitted light
  • External fluorescence lamp  LQ-HXP 120

Scanners (tandem scanner system)

  • Point-scanning
  • Zoom function
  • Scan field rotation
  • Regular scanner (field-of-view scanner): 1 Hz to 1800 Hz
  • Resonant scanner: 8 kHz
  • Two vario beam expanders for adjusting the IR beam diameter and divergence for optimal deep tissue imaging performance 
  • FRAP booster for higher single photon excitation laser powers at the sample

Objectives

Name Magnification NA Immersion WD (mm)
HC PL APO 10x/0.40 CS 10x 0.4 Air 2.2
HC PL APO 20x/0.75 IMM CORR CS2 20x 0.75 Multi-Immersion 0.66
HC IRAPO L 25x/1.0 W motCORR 25x 1.0 Water 2.6
HC FLUOTAR L 25x/1.0 IMM motCORR VISIR 25x 1.0 n=1.457 6
HC PL APO 63x/1.20 W motCORR CS2 63x 1.2 Water 0.3
HC PL APO 63x/1.40 Oil CS2 63x 1.4 Oil 0.14

Widefield Fluorescence Filters

  • DAPI
  • GFP (Installed)
  • CFP
  • DsRed
  • Polarisator for phase contrast

Detectors

  • 4 spectral (380-800nm) non-descanned FLIM capable photon-counting hybrid detectors (sm HyDs)
  • 4 spectral (380-800nm) descanned confocal detectors: one Photomultipliertube (PMT), two FLIM capable photon-counting hybrid detectors (sm HyDs) and one photon-counting hybrid detectors (HyD)
  • transmission light-channel (PMT-Trans)

FLIM Hardware

  • Fully integrated Leica fast lifetime contrast (FALCON) FLIM implementation
  • 4 non-descanned FLIM capable HyD detectors
  • 2 descanned FLIM capable HyD detectors
  • Single photon excitation with supercontinuum laser (Variable repetition rate)
  • Multiphoton excitation (80 MHz)
  • TCSPC modalities: FLIM / FLIM FRET / Phasor Analyis

Camera Systems

Leica DFC7000 T Color CCD Camera

  • digital color, high-sensitivity, cooled camera for fluorescence and brightfield microscopy
  • 1920x1440 pixel image size
  • RGB Bayer mask color filter
  • 4 microseconds - 200 seconds exposure
  • 8 or 12 bit bit depth
  • 40 fps @ full frame
  • color or monochrome operation

Accessories

  • Ludin light insulation box
  • Flexible Scientifica Motorised Movable Base Plate (MMBP) with accessories
  • CUDA accelerated inline Lightning deconvolution
  • Optional Leica SuperZ galvanometric z-stage for fast and precise z-positioning
  • Trigger box for triggering image acquisition or external hardware components

 

 

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

 

 

Literature and Links

Further information (internal UZH use only)

Follow this link for further background information, documents and links.

Responsible Persons

If you have questions about the microscope please contact Joana Raquel Delgado Martins.