Multiphoton - Leica TCS SP8 MP (Botinst)

image_mpe_botinst

The inverted Leica multiphoton and confocal fluorescence microscope is equipped with a pre-compensated infrared laser (Insight DS+ Dual from Spectra Physics) as well as with a whitelight laser for visible excitation. Besides multiphoton excitation as well as one photon excitation, non-linear experiments such as second harmonic generation can be performed with the system.

Location

BOT Room M2-21, Zollikerstrasse 107, 8008 Zürich. Phone +41 44 634 83 22

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Light Sources and Lasers

  • Insigth DS+ Dual (680-1300 nm & 1041nm) ultrafast NIR laser for multiphoton excitation
  • White light laser (WLL): continuous laser output in the range of 470-670nm; AOTF allows to choose up to 8 different wavelengths simultaneously; pulsed laser system with a repetition rate of 80 MHz
  • Diode laser 405 nm (50mW)
  • Argon Laser (65mW): 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
  • Halogen lamp for transmitted light
  • External fluorescence lamp  LQ-HXP 120

Scanners (tandem scanner system)

  • point-scanning
  • zoom function
  • Regular scanner (field-of-view scanner): 1 Hz to 1800 Hz
  • Resonant scanner: 8 kHz

Objectives

Name Magnification NA Immersion WD (mm)
HC PL APO 10x/0.40 CS2 10x 0.4 Air 2.2
HC PL APO 20x/0.75 IMM CORR CS2 20x 0.75 Multi-immersion 0.68
HC FLUOTAR L 25x/0.95 W VISIR 25x 0.95 Water 2.5
HC PL APO 63x/1.40 OIL CS2 63x 1.4 Oil 0.14
HC PL APO 63x/1.20 W motCORR CS2 63x 1.2 Water 0.3

Fluorescence Filters

Non-Descanned Detection:

  • HyD-RLD 2ch DAPI/FITC filter cube: 460/50 & 525/50 with 495 SP
  • HyD-RLD 2ch CFP/YFP filter cube: 483/32 & 535/30 with 505 SP
  • HyD-RLD 2ch TRITC/A633 filter cube: 585/40 & 650/50 with 620 SP
  • HyD-RLD 2ch A488/A647 filter cube: 525/50 & 675/55 with 620 SP

 

  • Beamsplitter Quad LP 505
  • Beamsplitter Quad SP 500
  • Beamsplitter Quad LP 560
  • Beamsplitter Quad SP 565

 

Widefield Fluorescence Filter Cubes

  • DAPI
  • GFP
  • CFP
  • DsRed

Detectors

  • 4 non-descanned super-sensitive photon-counting hybrid detectors (HyD)
  • 3 descanned confocal fluorescence channels: one Photomultipliertube (PMT) and two super-sensitive photon-counting hybrid detectors (HyD, suitable for time gated detection in combination with visible excitation)
  • transmission light-channel (PMT-Trans)

Camera Systems

Leica DFC7000 T Color CCD Camera

  • digital color, high-sensitivity, cooled camera for fluorescence and brightfield microscopy
  • 1920x1440 pixel image size
  • RGB Bayer mask color filter
  • 4 microseconds - 200 seconds exposure
  • 8 or 12 bit bit depth
  • 40 fps @ full frame
  • color or monochrome operation

Accessories

  • controlled environmental conditions (temperature, humidity)

 

 

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

 

 

Literature and Links

Further information (internal UZH use only)

Follow this link for further background information, documents and links.

Responsible Persons

If you have questions about the microscope please contact Joana Raquel Delgado Martins or José María Mateos Melero.