Widefield - Zeiss Axio Observer (Schlieren)

This inverted widefield system is suited to image live cells as well fixed fluorescence and histologically stained samples. A temperature and CO2 controlled environment is possible for life experiments.

Location

University Zurich, Schlieren, Room WAD-12-K-105. Phone +41 44 635 34 91

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope

  • Zeiss AxioObserver Z1

Light Sources

  • Lumencore SpectraX
  • Transmitted light halogen lamp

Objectives

Name Magnification NA Immersion          WD (mm)

EC Plan Neofluar (Ph1)

   5x

0.16

Air

18.5

EC Plan Neofluar (Ph1)   10x                             0.3 Air 5.2
LD Plan Neofluar (Ph2)   20x 0.4                   Air 7.4-8.4
LD LCI Plan Apochromat (DIC)   25x 0.8 IMM 0.57
LD Plan Neofluar (Ph2)   63x 0.75 Air 1.2-2.2
i-Plan Apochromat (DIC)   63 x 1.4 Oil 0.18

IMM = multi immersion (either water, glycerol or oil)

Ph = phase contrast

Fluorescence Filters

Regular fluorescence filters 

Name Excitation  Dichroic Emission
DAPI (violet) BP 390/22-25 395 BP 445/50
GFP (Cyan) BP484/25 495 BP 525/50
DsRed (Green) BP 553/18 570 BP 605/70
CY5 (Red) BP 631/22 660 BP 690/50
CFP (blue)BP 445/25460 BP 480/22
YFP(Teal)BP 513/22-25 520 LP 530
 

Default Setup contains DAPI, GFP, DsRed and CY5.  A QUADband filter (Zeiss 489081-9010) allows fast multi-channel recordings containing the 4 same channels.

Besides optional single filters for YFP and CFP, there is also an additional DOUBLEband YFP/CFP filter cube available.

Camera Systems

  • Hamamatsu Orca Flash 4.0 V2, sCMOS, cooled fluorescence camera 16bit, 2048 x 2048 pixel (4 MP), pixel size 6.5 µm

Accessories

  • Definite focus control
  • Temperature and CO2 control
  • isolation ring for 63x oil objective for optimized tempertaure control

Further information (internal UZH use only)

Follow this link for further background information, documents and links

System Status

 Follow this link for an updated status of the system

Responsible Persons

If you have questions about the microscope please contact Philipp Schätzle or Caroline Aemisegger.