CLSM - Leica SP8 inverse (Balgrist Campus)

The Leica SP8 is an automated inverse confocal laser scanning microscope allowing simultaneous aquisition of 3 fluorescent channels and 1 transmitted light channel (BF or DIC).

Location

University Zurich, Balgrist Campus, Lengghalde 5, 8008 Zurich, Room Microscopy W.112.

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Light sources and lasers

Halogen lamp for transmitted light
External fluorescence lamp LQ-HXP 120
Solid state diode lasers: 405 nm (50 mW), 488 nm (20 mW), 552 nm (20 mW) and 638 nm (30 mW)

Scanners

Dual scanning system:

Regular scanner: 10 - 1800 Hz

Resonant scanner: 8000 Hz

Objectives

Name	Magnification	NA	Immersion	WD (mm)
HC PL APO	10x	0.3	Air	11
HC PL APO CS2	20x	0.75	Imm	0.67
HCX PL APO 37°C CS2	63x	1.3	Glycerol	0.3

Detector System

Two Hybrid (HyD) detectors, one photomultiplier tube (PMT) detector and one additional PMT for transmission. Excitation controlled by AOTF, beam splitters: RT 15/85, substrate, TD 488/552/638, DD 488/552 for separation between excitation and emission. Accurate emission detection by spectral detectors.

Fluorescence Filters for widefield

Name	Excitation Range	Excitation Filter	Dichroic	Emission Filter
A4	UV	BP 360/40	400	BP 470/40
GFP	blue	BP 470/40	500	BP 525/50
N2.1	green	BP 515-560	580	LP 590
CFP	violet/blue	BP 436/20	455	BP 480/40
I3	blue	BP 450/490	410	LP 515

Available Accessories

controlled environmental conditions (CO₂, temperature, humidity)
Matrix screening software (reservation needed)

Remarks

This microscope acquires images with improved axial resolution compared with more coventional widefield microscopes. In addition to the multicolor images the microscope can acquire large tile scans fully automatically. The microscope allows live imaging with controlled environmental conditions (CO₂, temperature, humidity).