CLSM - Leica Stellaris 5 inverse (Bot)

The Leica STELLARIS 5 is an advanced automated inverse confocal laser scanning microscope. It offers the capability of simultaneously capturing three distinct fluorescent channels alongside a high-quality transmission bright-field channel. Featuring the cutting-edge TauSense technology, it provides real-time access to lifetime-based data, enriching your experiments with valuable insights. This system is particularly well-suited for examining fixed samples

Location

BOT Room M2-19, Zollikerstrasse 107, 8008 Zürich.

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Light sources and lasers

Halogen lamp for transmitted light
External fluorescence light source - Lumencor LED 3
405 nm diode laser
Leica white light laser (WLL - 485 nm to 685 nm excitation)

Scanners

Dual scanning system:

Regular scanner: 1 - 2600 Hz

Resonant scanner: 8000 Hz

Objectives

Name	Magnification	NA	Immersion	WD (mm)
HC PL APO CS2	10	0.4	Air	2.56
HC PL APO CS2	20x	0.75	Air	0.62
HC PL APO CS2	40x	1.25	Glycerol	0.35
HC PL APO CS2	63x	1.4	Oil	0.14
HC PL APO CS2	63x	1.3	Glycerol	0.3

^{CS2 objectives: improved color correction, perfect VIS-405; IMM = multi immersion (either water, glycerol or oil)}

Detector System

3 Hybrid (Power HyD S) detectors, and one additional detector for transmission (Trans PMT). Excitation controlled by AOTF. Accurate emission detection by spectral detectors.

Fluorescence Filters

Name	Excitation Range	Excitation Filter	Dichroic	Emission Filter
LED_405	UV	BP 405/60	455	BP 470/40
GFP	blue	BP 470/40	495	BP 525/50
RHOD	green	BP 546/10	560	BP 585/40

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

Remarks

This microscope offers enhanced axial resolution compared to traditional widefield microscopes. With the latest TauSense technology, it provides immediate access to lifetime-based data, augmenting the depth of information available for your experiments. Furthermore, in addition to capturing multi-color images in fixed samples, the microscope can fully automate the acquisition of large tile scans, all thanks to the LAS X Navigator.