CLSM - Leica Stellaris 5 inverse (USZ)

The Leica STELLARIS 5 is an automated inverse confocal laser scanning microscope allowing simultaneous acquisition of 3 fluorescent channels as well as a transmission bright field channel. The newest TauSense technology gives instant access to lifetime-based information, delivering additional insights to your experiments.This system is ideal for fixed samples.

Location

University Zurich Center, at the USZ Schmelzbergstrasse 12, Room PATH A25.

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Light sources and lasers

Halogen lamp for transmitted light
External fluorescence light source - Lumencor LED 3
405 nm diode laser
Leica white light laser (WLL - 485 nm to 685 nm excitation)

Scanners

Regular scanner: 1 - 2600 Hz

Objectives

Name	Magnification	NA	Immersion	WD (mm)
HC PL FLUOTAR	5	0.15	Air	13.70
HC PL APO CS2	10	0.4	Air	2.56
HC PL APO CS2	20x	0.75	Air	0.62
HC PL APO CS2	20x	0.70	IMM	0.26-0.17
HC PL APO CS2	63x	1.4	Oil	0.14

^{CS2 objectives: improved color correction, perfect VIS-405; IMM = multi immersion (either water, glycerol or oil)}

Detector System

3 Hybrid (Power HyD S) detectors, and one additional detector for transmission (Trans PMT). Excitation controlled by AOTF. Accurate emission detection by spectral detectors.

Fluorescence Filters

Name	Excitation Range	Excitation Filter	Dichroic	Emission Filter
DAPI.	UV	BP 405/60	455	BP 470/40
GFP	blue	BP 470/40	495	BP 525/50
RHOD	green	BP 546/10	560	BP 585/40

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

Remarks

This microscope acquires images with improved axial resolution compared with more coventional widefield microscopes.The newest TauSense technology gives instant access to lifetime-based information, delivering additional insights to your experiments. In addition to the multicolor images in fixed samples the microscope can acquire large tile scans fully automated thanks to the LAS X Navigator.