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Center for Microscopy and Image Analysis

HCS - MD ImageXpress Confocal HT.ai

ImageXpress Confocal HT.ai

The ImageXpress Confocal HT.ai High-Content imaging system utilizes a seven-channel laser light source (410 nm - 750 nm) with eight imaging channels to enable highly multiplexed assays. Designed for experiments involving live cells as the spinning disk reduces phototoxicity and enables fast acquisition. The system supports slides and one to 1536-well microplates, round or flat bottom, low to high profile.

Location

University Zurich, Irchel Campus, Room Y42-H-91. 

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope

  • inverted microscope (ImageXpress Confocal HT.ai)
  • Precision motorized X-Y (sample) stage

  • Dual disk unit allowing selection between 3 modes:

    • 50 micron pinhole disk for your basic confocal requirements (better optical sectioning but less sensitive). Spacing between pinholes 500 um

    • 50 micron (named 51 micron slit) for high throughput confocal requirements (faster acquisition but lower optical sectioning). Spacing between pinholes 250 um

    • Widefield (not-confocal) imaging mode

Objectives

Default installed objectives

            
Name Mag NA Immersion WD (mm) Pixel size (um/pixel)

Recommended z step for deconvolution (um)
Nikon LWD Lambda S 40XC WI 40x 1.15 Water 0.61–0.59

0.1687  

 0.2 
Nikon CFI Apo LWD Lambda S 20XC WI 20x 0.95 Water 0.99–0.9 0.344 0.3
Nikon CFI Plan Apo Lambda 10X 10x 0.45 Air 4 0.678  
Nikon CFI Plan Apo Lambda 40XC 40x 0.95 Air 0.25–0.17    
     
Optional objectives to be installed on request             
Nikon CFI Plan Apo Lambda 20X 20x 0.75 Air 1

 

  
Nikon CFI Plan Apo Lambda 2X 2x 0.10 Air 8.5    
             

Laser light source specifications

Channel Excitation Laser (nm) Common Fluorophores Emission Filter
DAPI  405/20 DAPI, Hoechst, Alexa Fluor 350, BFP 452/45 nm
CFP  445/20 CFP 483/32 nm
FITC  470/21 FITC, GFP, FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green 520/28 nm
YFP  520/10 YFP 562/40 nm
TRITC 555 Cy3, TRITC, Alexa Fluor 532 & 546 & 555, Rhodamine, TAMRA, LysoTracker Yellow, MitoTracker Orange

598/25 nm
Texas Red 555 Texas Red, mCherry, Alexa Fluor 568 & 594, Calcium Crimson, Cy3.5, HcRed, MitoTracker Red, Propidium Iodide 624/40 nm
Cy5  638/17 Cy5, Alexa Fluor 647 & 660, DRAQ5, APC (Allophycocyanin), BODIPY 650/665, DiD, SYTO Red, TOTO-3, and TO-PRO-3 692/40 nm
Cy7  725/30 Cy7 794/32
ImageXpress Confocal HT.ai

Camera Systems

  • high-quantum efficiency 16-bit, >4 megapixel scientific CMOS sensor

Accessories

  • Inserts for ibidi slides and well plates
  • Incubation system for live cell imaging (ImageXpress)
  • Allows for a variety of experimental protocols with 0 - 20 % CO2, 0 - 100% nitrogen, 0 - 100% Oxygen.
  • IN Carta Image Analysis Software

Literature and Links

Guides - MD ImageXpress

ibiology - Optical sectioning and confocal microscopy

Spinning Disk Confocal Microscopy

 

Responsible Persons

If you have questions about the device please contact the responsible person.

 

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

Recommended imaging supports

In order to ensure reliable imaging at this instrument we recommend using imaging supports with high performance #1.5 cover glass or equivalent. The following imaging supports have been successfully used:

 

If you'd like to ask for a free sample to test, please use the link https://ibidi.com/content/875-ibidi-free-sample-program

We do not support or troubleshoot autofocus strategies using ultra-thin transparent film bottoms. 

Please contact us in case you have further question. 

 

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