HCS - Perkin Elmer Operetta (Lengg)
From everyday assays to more demanding applications, the Operetta CLS (spinning disk) high-content analysis system delivers just the right combination of flexible excitation, sensitive optics, and advanced software features to enable you to gain deeper biological insight from all your critical applications.
Location
University of Zurich, Forschungszentrum für das Kind FZK, August-Forel-Strasse 51, 8008 Zürich, room: LLF.C+4.020
Training Request
Follow this link to apply for an introduction to the microscope.
Technical Specifications
Microscope
-
Precisicon autofocus unit
-
Dual Nipkow spinning disk for optical sections
-
Widefield (not-confocal) imaging mode
Objectives
|
Installed objectives |
|||||
|---|---|---|---|---|---|
| Name | Mag | NA | Immersion | WD (mm) | Field of View (µm2) |
|
Opera PHX/OPRTCLS HH14000422 |
40x | 1.1 | Water | 0.62 |
338 x 254 |
|
Opera PHX/OPRTCLS HH14000421 |
20x | 1.0 | Water | 1.7 | 646 x 646 |
| DACH CLS | 20x | 0.4 | Air | 8.28 | 646 x 646 |
|
Opera PHX/OPRTCLS HH14000403 |
10x | 0.3 | Air | 5.2 | 1292 x 1292 |
|
Opera PHX/OPRTCLS HH14000402 |
5x
|
0.16 | Air | 12.1 |
2583 x 2583
|
LED Excitation Light
| Excitation Laser (nm) | Common Fluorophores | Emission Filter (nm) |
|---|---|---|
| 365 | Live/Dead Fix Aqua, Marina Blue, Fura-2 | 360-400 |
| 405 | DAPI, Hoechst, Alexa Fluor 350, BFP | 460-490 |
| 440 | CF440, ER-ID Green | 500-550 |
| 475 | FITC, GFP, FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green | 570-650 |
| 510 | YFP | 570-650 |
| 550 | Cy3, TRITC, Alexa Fluor 532 & 546 & 555, Rhodamine, TAMRA, LysoTracker Yellow, MitoTracker Orange | 570-650 |
| 550 | Texas Red, mCherry, Alexa Fluor 568 & 594, Calcium Crimson, Cy3.5, HcRed, MitoTracker Red, Propidium Iodide | 655-760 |
| 630 | Cy5, Alexa Fluor 647 & 660, DRAQ5, APC (Allophycocyanin), BODIPY 650/665, DiD, SYTO Red, TOTO-3, and TO-PRO-3 | 655-760 |
| 660 | Cy7 | 655-760 |
Camera Systems
- sCMOS camera with 4.7 megapixels (2160 x 2160), 16 bit resoltuion and 6.5 µm pixel size
Accessories
- 4 slide sample holder
- Climate control Opera PHX/OPRTCLS: The sample chamber is equipped with temperature and CO2 control for live cell experiments. The temperature can be regulated between 37°C and 42°C and the CO2 concentration between 1% and 10%
Literature and Links
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy
Recommended imaging supports
In order to ensure reliable imaging at this instrument we recommend using imaging supports with high performance #1.5 cover glass or equivalent. The following imaging supports have been successfully used:
- 96 well plate ibidi - P96-1.5H-N (#1.5 glass bottom, Cat.No:89627)
- 96 well plate ibidi - P96-1.5P (#1.5 glass-like polymer coverslip bottom, Cat.No:89626)
If you'd like to ask for a free sample to test, please use the link https://ibidi.com/content/875-ibidi-free-sample-program
We do not support or troubleshoot autofocus strategies using ultra-thin transparent film bottoms.
Please contact us in case you have further question.