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Center for Microscopy and Image Analysis

Spinning Disk- Olympus (Irchel)

Spinning disk Olympus

The Olympus IXplore SpinSR10 super resolution imaging system is a high-end real time spinning disk confocal imaging system. 

It is equipped with two sCMOS cameras, which allow fast and sensitive simultaneous acquisition of two-color labelled (live) samples.

Designed for experiments involving live cells, the spinning disk (YOKOGAWA CSU-W1) reduces phototoxicity and bleaching while the Olympus Z-drift compensator (IX3-ZDC2) maintains focus.

The photomanipulation unit (Rapp OptoElectronic) allows localized and controlled photostimulation as well as damage/ablation in biological samples. 

The stage top incubation cellVivo system allows control of temperature and CO2 levels enabling live cell experiments.

Location

University Zurich, Irchel Campus, Room Y42-H-83. 

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope

  • inverted microscope (Olympus Inverted IX83)
  • Yokogawa CSU-W1 (2 discs: 50 μm pinhole disk for high resolution confocal imaging, SoRa disk for super resolution imaging)
  • motorized xyz stage (IX3-SSU)
  • Olympus real-time controller (U-RTCE)
  • Olympus Z-drift compensator (IX3-ZDC2)

Light Sources and Lasers

  • Laser lines: 405 nm (50 mW), 488 (100mW), 561 nm (100 mW), 640nm (100mW), 445nm (75nm) and 514nm (40mW).
  • Photomanipulation unit: 355 nm (pulsed, UGA‑42 Caliburn), 405 nm (100 mW), 473 nm (100mW)
  • CoolLED’s pE-300 LED illumination for widefield fluorescence microscopy (3 LEDs). Spectral coverage from the UV (DAPI excitation) to the red region (Cy5 excitation)

Objectives

Name Magnification NA Immersion WD (mm)
UPLSAPO UPlan 4x 0.16 Air 13
UPLXAPO20X 20x 0.8 Air 0.6
UPLAN S Apo 40x 0.95 Air 0.18

Silicon oil Immersion

         
UPLSAPO UPlan S Apo 30x 1.05 Silicon oil 0.8

UPLSAPO UPlan S Apo

 60x 1.3

Silicon oil

 0.3

UPLSAPO UPlan S Apo

 100x 1.3

Silicon oil

 0.2

Additional objectives (not currently installed)

Name Magnification NA Immersion WD (mm)
LUCPLFN PH 20x 0.45 Air

6.60 - 7.80

 

Fluorescence Filters

Confocal Imaging Filters (implemented in CSU-W1)

CSU-W1

Recommended Imaging Settings

Name/ Excitation Lasers
Image splitting dichroic
 Camera / Wheel 1 Camera / Wheel 2
405/561 A561 LP BP 617/73 BP 447/60
488/640 A561 LP BP 685/40 BP 525/50
405/488 A514 LP BP 525/50 BP 447/60
405 Glass BP 447/60 -
488 Glass BP 525/50 -
561 Glass BP 617/73 -
640 Glass BP 685/40 -
445 Glass BP 482/35 -
514 Glass BP 578/105 -

Widefield

For further details please contact the responsible persons.

 

Camera Systems

  • 2x Hamamatsu ORCA-Fusion sCMOS
  • 2304 x 2304 pixel (6.5 x 6.5um pixel size)
  • up to 89.1 fps @ 16-bit (at 2304 x 2304 pixel)

Accessories

  • Inserts for ibidi slides, well plates and 35mm dishes
  • Incubation system for live cell imaging (cellVIVO)
  • Allows for a variety of experimental protocols with 0 - 20 % CO2.
  • Reliable control of temperature and CO2 with up to 0.05 °C accuracy

Literature and Links

Guides - Spinning disk Olympus

ibiology - Optical sectioning and confocal microscopy

Spinning Disk Confocal Microscopy

 

Responsible Persons

If you have questions about the device please contact the responsible person.

 

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

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