Lightsheet - Zeiss Z.1 (Irchel) | Center for Microscopy and Image Analysis

The Zeiss Z.1 lightsheet fluorescence microscope enables long term imaging of large biological samples. It delivers optical sections of large samples, with virtually no phototoxicity or bleaching and with high temporal resolution. The lightsheet microscope is suited to study biological and developmental processes of entire living systems such as zebrafish embryos, drosophila larva or plants with cellular resolution.

Location

University Zurich, Irchel Campus, Room Y42-H-91.

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Light Sources and Lasers

Solid state diode lasers: 405nm (20mW), 445nm (25mW), 488nm (50mW), 561nm (50mW), 638nm (75mW)

Objectives

Name	Magnification	NA	Immersion	WD (mm)
EC Plan-NEOFLUAR	5x	0.16	Air	18.5
Plan APO	10x	0.5	Water	3.7
W Plan APO	20x	1.0	Water	2.4
W Plan APO	40x	1.0	Water	2.5
W Plan-APO	63x	1.0	Water	2.1

Fluorescence Filters

Filter wheel 1

Name (Wheel Pos.)	Dichroic	Camera 1	Camera 2
green, red (P5)	lp 560	sp 550	lp 585
gfp, mcherry (P6)	lp 560	bp 505-530	lp 585
dapi, draq5 (P2)	lp 490	bp 420-470	lp 660
dapi, cy3 (P3)	lp 510	bp 420-470	bp 575-615
cfp, draq5 (P4)	lp 510	bp 460-500	lp 660

Filter wheel 2

Name (Wheel Pos.)	Dichroic	Camera 1	Camera 2
dapi, gfp (P2)	lp 490	bp 420-470	bp 505-545
dapi, cy3 (P3)	lp 510	bp 420-470	bp 575-615
gfp, cy3 (P4)	lp 560	bp 505-545	bp 575-615
gfp, draq5 (P5)	lp 560	bp 505-545	lp 660
cfp, cy3 (P6)	lp 510	bp 460-500	bp 575-615

Camera Systems

Two sCMOs-Cameras: 3.8 MPixel (pixel size 6.5 µM), maximal frame rate 30 fps at 960 x 1024 pixel.

The acquisition speed of the Z.1 microscope is strongly dependent on the chosen number of cameras and resolution. For achieving high temporal resolution, this parameters have to be optimized carefully:

1 Camera Active (1 Color Experiment)

2 Cameras Active (2 Color Experiment)

Field of View

Speed

Field of View

Speed

1920 x 1920

20 fps

1920 x 1920

10 fps

960 x 960

57 fps

960 x 960

36 fps

800 x 800

57 fps

800 x 800

50 fps

640 x 640

67 fps

640x 640

67 fps

>96-480 x 96

80 fps

>96-480 x 96

80 fps

Accessories

Controlled environmental conditions (CO2, temperature)

Remarks

For initial tests and the first experiments, the ZMB will serve users with the needed experimental accessories such as syringes, tubing and capillaries. If they become regular user of the microscope, they have to organize the accessories by themselves.
Lightsheet microscopy is a very powerful technique which allows performing prolonged experiments with high temporal and special resolution. It is possible to produce very large amounts of data within short periods of time (>50 Gigabyte per minute). Due to the very limited storage capacity at the ZMB, lightsheet users are encouraged to think about data storage and processing from the very beginning of their experiments.

Literature and Links

http://www.zeiss.com/microscopy/en_de/products/imaging-systems/lightsheet-z-1.html

http://zeiss-campus.magnet.fsu.edu/referencelibrary/lightsheet.html

Further information (internal UZH use only)

Follow this link for further background information, documents and links.

Responsible Persons

If you have questions about the device please contact the responsible person.

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

Center for Microscopy and Image Analysis

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Lightsheet - Zeiss Z.1 (Irchel)