Lightsheet - Zeiss Z.1 (Irchel) - System Discontinued!
System Discontinued
This system has been permanently discontinued and is no longer available. For further assistance or information about alternative solutions, please contact micoscopy@zmb.uzh.ch
The Zeiss Z.1 lightsheet fluorescence microscope enables long term imaging of large biological samples. It delivers optical sections of large samples, with virtually no phototoxicity or bleaching and with high temporal resolution. The lightsheet microscope is suited to study biological and developmental processes of entire living systems such as zebrafish embryos, drosophila larva or plants with cellular resolution.
Location
University Zurich, Irchel Campus, Room Y42-H-91.
Training Request
Follow this link to apply for an introduction to the microscope.
Technical Specifications
Light Sources and Lasers
Solid state diode lasers: 405nm (20mW), 445nm (25mW), 488nm (50mW), 561nm (50mW), 638nm (75mW)
Objectives
| Name | Magnification | NA | Immersion | WD (mm) | |
|---|---|---|---|---|---|
| EC Plan-NEOFLUAR | 5x | 0.16 | Air | 18.5 | |
| Plan APO | 10x | 0.5 | Water | 3.7 | |
| W Plan APO | 20x | 1.0 | Water | 2.4 | |
| W Plan APO | 40x | 1.0 | Water | 2.5 | |
| W Plan-APO | 63x | 1.0 | Water | 2.1 |
Fluorescence Filters
Filter wheel 1
| Name (Wheel Pos.) | Dichroic | Camera 1 | Camera 2 |
|---|---|---|---|
| green, red (P5) | lp 560 | sp 550 | lp 585 |
| gfp, mcherry (P6) | lp 560 | bp 505-530 | lp 585 |
| dapi, draq5 (P2) | lp 490 | bp 420-470 | lp 660 |
| dapi, cy3 (P3) | lp 510 | bp 420-470 | bp 575-615 |
| cfp, draq5 (P4) | lp 510 | bp 460-500 | lp 660 |
Filter wheel 2
| Name (Wheel Pos.) | Dichroic | Camera 1 | Camera 2 |
|---|---|---|---|
| dapi, gfp (P2) | lp 490 | bp 420-470 | bp 505-545 |
| dapi, cy3 (P3) | lp 510 | bp 420-470 | bp 575-615 |
| gfp, cy3 (P4) | lp 560 | bp 505-545 | bp 575-615 |
| gfp, draq5 (P5) | lp 560 | bp 505-545 | lp 660 |
| cfp, cy3 (P6) | lp 510 | bp 460-500 | bp 575-615 |
Camera Systems
Two sCMOs-Cameras: 3.8 MPixel (pixel size 6.5 µM), maximal frame rate 30 fps at 960 x 1024 pixel.
The acquisition speed of the Z.1 microscope is strongly dependent on the chosen number of cameras and resolution. For achieving high temporal resolution, this parameters have to be optimized carefully:
| 1 Camera Active (1 Color Experiment) | 2 Cameras Active (2 Color Experiment) | ||
| Field of View | Speed | Field of View | Speed |
| 1920 x 1920 | 20 fps | 1920 x 1920 | 10 fps |
| 960 x 960 | 57 fps | 960 x 960 | 36 fps |
| 800 x 800 | 57 fps | 800 x 800 | 50 fps |
| 640 x 640 | 67 fps | 640x 640 | 67 fps |
| >96-480 x 96 | 80 fps | >96-480 x 96 | 80 fps |
Accessories
Controlled environmental conditions (CO2, temperature)
Remarks
- For initial tests and the first experiments, the ZMB will serve users with the needed experimental accessories such as syringes, tubing and capillaries. If they become regular user of the microscope, they have to organize the accessories by themselves.
- Lightsheet microscopy is a very powerful technique which allows performing prolonged experiments with high temporal and special resolution. It is possible to produce very large amounts of data within short periods of time (>50 Gigabyte per minute). Due to the very limited storage capacity at the ZMB, lightsheet users are encouraged to think about data storage and processing from the very beginning of their experiments.
Literature and Links
Further information (internal UZH use only)
Follow this link for further background information, documents and links.
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy