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Center for Microscopy and Image Analysis

Widefield - Olympus ScanR HCS (Irchel)

ScanR

ScanR is a modular microscope-based imaging platform designed for fully automated image acquisition and data analysis of biological samples. ScanR can handle many different formats, including multi-well plates, slides and custom-built arrays. This unmatched flexibility makes it equally adept at routine and advanced applications.

 

Location

University Zurich, Irchel Campus, Room Y24-F-14.

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope

Motorized Olympus high-end research microscope IX83 with motorized Z-drive.

Light Sources

  • High power LED illumination system: Lumencor SpectraX light engine.

Objectives

Name Magnification NA Immersion WD (mm)
UPLSAPO 4x 0.16 Air 13
UPLSAPO 10x 0.4 Air 3.1
UPLSAPO 20x 0.75 Air 0.6
UPLSAPO 40x 0.9 Air 0.18
UPLFLN 10x Phase 0.3 Air 10
PLAPON 60x Phase 1.42 Oil 0.15

Fluorescence Filters

Fluorescence filters (Fast emission filter wheel)

Name Excitation  Dichroic Emission
DAPI SEM BP 395/25 400 BP 435/26
FITC SEM BP 470/24 485 BP 515/30
TRITC SEM BP 550/15 558 BP 595/40
CY5 SEM BP 640/30 660 BP 705/72

Camera Systems

  • Hamamatsu ORCA-FLASH 4.0 V2, high-sensitivity digital monochrome scientific cooled sCMOS camera, QE > 70% @ 600 nm, dynamic range 4000:1, 2048 x 2948 pixel of size 6.5 x 6.5 um, 12 bit dynamics, max. exposure time 10s. (recommended for low signal levels and high field-of-view applications).

Accessories

  • Controlled environmental conditions (CO2, temperature) CellVivo incubation system
  • Multiwell insert
  • ScanR Analysis software: Automated image and data analysis for standard assays and assay development. Online and offline multicore analysis. Image processing, image analysis, particle detection, and parameter extraction and calculation. Cytometric data exploration, analysis, gating, and classification
    Powerful and flexible gating concept including automated population detection
    Direct link between data points, objects, and images
    Assays-based work flow and advanced scientific assay development functionality

 

Responsible Persons

If you have questions about the device please contact the responsible person.

 

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

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