Navigation auf uzh.ch

Suche

Center for Microscopy and Image Analysis

Widefield - GE IN Cell Analyzer 2500HS (Irchel)

GE InCell

 

The INCell Analyzer 2500HS is a fully-automated widefield fluorescence imaging system dedicated to life cell imaging and high content and high throughput imaging. The system allows for sample automation using a robot as well as for liquid handling for drug applications and water immersion.

Location

University Zurich, Irchel Campus, Room Y42-H-91. 

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope

  • GE inverted microscope.

Light Sources

  • Seven-color Solide State Illuminator (SSI) for fluorescence excitation
  • White LED for transmitted light

 

Name Magnification NA Immersion          WD (mm)
CFI Plan Apo Lambda   10x                             0.45 Air 4.0
CFI Plan Apo Lambda   20x 0.75               Air 1.0
CFI Plan Apo Lambda   40x 0.95 Air 0.2-0.11

CFI Plan Apo Lambda*

   40x 1.15 Water 0.61

*Automated water immersion system is now fully supported in InCell software.

Fluorescence Filters

IN Cell Analyzer 2500HS is equipped with two quad band pass polychroic mirrors and single band pass emission filters.

Polychroic beam splitter BGOFR_1

Filter name

 Excitation  Emission
Blue BP 390/18 BP 432.5/47
Green_1 BP 475/28 BP 511.5/23
Orange BP 542/27 BP 587.5/47
FarRed BP 632/22 BP 676/48
Polychroic beam splitter BGRFR_2

Filter name

 Excitation  Emission
Blue BP 390/18 BP 432.5/47
Green_2 BP 475/28 BP 526/52
Red BP 575/25 BP 607.5/19
FarRed BP 632/22 BP 676/48

Camera Systems

  • PCO - sCMOS, 16 bit, 2048 x 2048 pixel, pixel size 6.5 µm, readout speed: 272 MHz

Imaging Modes

  • Transmitted light, DIC (digital), Phase (digital)
  • 2D deconvolution, Advanced 2D deconvolution, 2.5D, 3D deconvolution

Accessories

  • Complete environmental control (temperature, CO2, N2, humidity)

  • Sample holders for multiwell plates, regular slides (1 or 4 pieces), chambered coverglass slides, 35 mm dishes
  • liquid handling
  • robotic automation

Remarks

Templates for slides

In order to facilitate the imaging process of slides with the GE IN CellAnalyzer we recommend to prepare the slides according to the templates depicted in the following document GE templates (PDF, 308 KB). For each of these slide-setups you will find a predefined slide profile in the GE software.

Literature and Links

Follow this link for further background information, documents and links (internal UZH use only).

 

 

Responsible Persons

If you have questions about the device please contact the responsible person.

 

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

Weiterführende Informationen

News

Bereichs-Navigation

Unterseiten von Widefield - GE IN Cell Analyzer 2500HS (Irchel)