Navigation auf uzh.ch
Navigation auf uzh.ch
This inverted widefield system is suited to image live cells as well fixed fluorescence and histologically stained samples. A temperature and CO2 controlled environment is possible for life experiments.
University Zurich, Schlieren, Room WAD-12-K-105.
Follow this link to apply for an introduction to the microscope.
Microscope
| Name | Magnification | NA | Immersion | WD (mm) |
|---|---|---|---|---|
|
EC Plan Neofluar (Ph1) |
5x |
0.16 |
Air |
18.5 |
| EC Plan Neofluar (Ph1) | 10x | 0.3 | Air | 5.2 |
| LD Plan Neofluar (Ph2) | 20x | 0.4 | Air | 7.4-8.4 |
| LD LCI Plan Apochromat (DIC) | 25x | 0.8 | IMM | 0.57 |
| LD Plan Neofluar (Ph2) | 63x | 0.75 | Air | 1.2-2.2 |
| i-Plan Apochromat (DIC) | 63 x | 1.4 | Oil | 0.18 |
IMM = multi immersion (either water, glycerol or oil)
Ph = phase contrast
| Name | Excitation | Dichroic | Emission |
|---|---|---|---|
| DAPI (violet) | BP 390/22-25 | 395 | BP 445/50 |
| GFP (Cyan) | BP484/25 | 495 | BP 525/50 |
| DsRed (Green) | BP 553/18 | 570 | BP 605/70 |
| CY5 (Red) | BP 631/22 | 660 | BP 690/50 |
| CFP (blue) | BP 445/25 | 460 | BP 480/22 |
| YFP(Teal) | BP 513/22-25 | 520 | LP 530 |
Default Setup contains DAPI, GFP, DsRed and CY5. A QUADband filter (Zeiss 489081-9010) allows fast multi-channel recordings containing the 4 same channels.
Besides optional single filters for YFP and CFP, there is also an additional DOUBLEband YFP/CFP filter cube available.
Follow this link for further background information, documents and links
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy
