Fluigent Aria (Irchel)

Fluigent Aria (Irchel, Schlieren, Botanical Garden, Lengg)

The Fluigent Aria system is an advanced microfluidic flow controller designed to automate and optimize fluid delivery for various microscopy applications, including multiplexed imaging, automated immunolabeling and DNA-PAINT workflows. Its precise pressure-based control allows reliable reagent delivery, minimizing sample loss while ensuring reproducibility. The Aria is particularly well-suited for applications requiring multi-channel fluid handling, such as spatial biology, live-cell imaging, and tissue staining.

Automated Fluid Delivery: Controls up to 10 fluidic channels for sequential or parallel reagent delivery.
Precision Flow Control: Pressure-based regulation ensures stable flow rates with ±5% accuracy and ±0.5% repeatability.
Compatible with Imaging Systems: Seamless integration with TTL-enabled widefield and confocal microscopes.
Minimal Reagent Waste: Low dead volume (36 µl) and customizable flow paths reduce sample and reagent consumption.
Reproducible Results: Automated protocols eliminate manual pipetting variability.
Flexible Integration: Supports microfluidic chips, perfusion chambers, and tissue slides.

Responsible Person	responsible person
Location	University Zurich, Irchel Campus, Room Y38-M-77.
Training Request	Follow this link to apply for an introduction to the microscope

: Zoom

Applications

cyclic multiplexing
automated immunolabeling
Exchange-PAINT microscopy

Technical Specifications

Channel Configuration	Supports up to 9 independent fluidic channels with flexible control + 1 line for waste.
M-Switch Valve	Multi-channel output for applications requiring multiple flow paths, such as perfusing multiple chips simultaneously.
Flow Rate Range	40 µL/min to 1 mL/min (Accuracy: ±5% above 40 µL/min. and Repeatability: ±0.5%.)
Pressure Control	Regulated up to 2.4 bar (34.8 psi)
Stop Flow Functionality	Integrated on-off valve to halt flow instantly during imaging or incubation steps.
Wetted Surfaces	Polypropylene, FEP, Glass, PEEK, and other materials ensure compatibility with most aqueous solutions and buffers.
Reservoirs	Standard: 8 × 15 mL (FALCON cat. 352096) Convertible: Adapters available for 2 mL reservoirs (Eppendorf). Flushing Reservoir: 2 × 100 mL for Buffers (Duran cat. 10153744)
Tubing	Material: FEP (1/16" OD, 250 µm ID). Chemical Compatibility: All reagents must be compatible with wetted surfaces (Polypropylene, FEP, Glass, PEEK). Avoid corrosive or caustic substances.
Fluidic accessoires	microfluidic-chipshop
Reccommended chips	Ibidi sticky tissue ibidi 6 channel slides glass bottom
User Guide	Aria Guides ARIA Tutorial Episode 1 : How To Plug An ARIA ARIA Tutorial Episode 2 : Connect To Set The Fluidic Path ARIA Tutorial Episode 3 : How To Fill Up The Reservoirs ARIA Tutorial Episode 4 : How To Use ARIA Manually ARIA Tutorial Episode 5 : Perform A Calibration With ARIA ARIA Tutorial Episode 6 : Global Handling Of ARIA ARIA Tutorial Episode 7 : Software Presentation
Links & Literature	Fluigent Aria
Aria User manual	Download Aria User Manual
Aria Software Developement Kit	Download Aria Software Development Kit Manual
Microfluidics on Scope	Microfluidcs on Scope
Fully automated TTL signal connection	TTL Connection Examples
Automated IBEX via TTL Leica	Automated IBEX via TTL

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Fluigent Aria (Irchel)