Lunaphore COMET (Schlieren)
Lunaphore COMET
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The Lunaphore COMET system is a fully automated, high-throughput multiplex immunofluorescence platform designed for spatial biology and tissue profiling. By integrating microfluidics, staining, and imaging in one streamlined workflow, COMET enables the analysis of multiple biomarkers (40+ plex) on a single tissue section without compromising sample integrity.
Responsible Person |
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Location |
University Zurich, Schlieren Campus, Room WAD-12-K-105. |
Training Request |
Follow this link to apply for an introduction to the microscope |
Applications |
Multiplex sequential immunofluorescence (seqIF) and RNAscope HiPlex |
| Plex-level |
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Technical Specifications
Slide capacity |
4 slides |
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Slide compatibility |
Standard histology slides - 75 mm x 25 mm (3 in x 1 in), 1 mm thick, positively charged such as Epredia SuperFrost Plus |
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Sample fixation |
Optimized for FFPE samples and frozen sections | |
Sample thickness |
3-10 μm | |
Automation level |
Fully automated: staining, target probe hybridization, image acquisition and image preprocessing | |
Maximum imaging area |
12.5 mm x 12.5 mm | |
Maximum staining area |
21 mm x 21 mm | |
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Throughput |
Measured for a 9 mm x 9 mm imaging area: 20 slides/week for 20-plex protein panel ; 12 slides/week for 40-plex protein panel | |
Reagents |
Open choice of primary antibodies (non-conjugated): |
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Software |
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Available Optics |
20X / 0.7 NA | |
Image resolution |
0.28 µm/pixel | |
Available Channels |
DAPI, FITC, TRITC, Cy5, Cy7 | |
Output file format |
OME-TIFF | |
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User Guide |
COMET USER Guide | |
Links & Literature |
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Reagents
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Application |
Reagent/Item |
Cat. No. |
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RNAscope HiPlex Pro for COMET 12-plex Kit |
RNAscope HiPlex Pro AMP 1 |
ACD (Bio-Techne) 322075 |
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RNAscope HiPlex Pro AMP 2 |
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RNAscope HiPlex Pro AMP 3 |
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RNAscope HiPlex Pro Fluoro T1-T4 |
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RNAscope HiPlex Pro Fluoro T5-T8 |
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RNAscope HiPlex Pro Fluoro T9-T12 |
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RNAscope HiPlex PretreatPro™ |
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RNAscope HiPlex Pro Probe Diluent |
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RNAscope HiPlex Pro DAPI (1 mg/mL) |
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RNAscope HiPlex Cleaving Stock Solution v2 |
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RNAscope HiPlex Pro Imaging Buffer Part A |
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RNAscope HiPlex Pro Imaging Buffer Part B |
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RNAscope HiPlex Pro for COMET 12-plex Kit |
RNAscope HiPlex Pro AMP 1 |
ACD (Bio-Techne) 322075 |
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RNAscope HiPlex Pro AMP 2 |
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RNAscope HiPlex Pro AMP 3 |
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RNAscope HiPlex Pro Fluoro T1-T4 |
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RNAscope HiPlex PretreatPro™ |
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RNAscope HiPlex Pro Probe Diluent |
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RNAscope HiPlex Pro DAPI (1 mg/mL) |
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RNAscope HiPlex Cleaving Stock Solution v2 |
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RNAscope HiPlex Pro Imaging Buffer Part A |
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RNAscope HiPlex Pro Imaging Buffer Part B |
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Control Probes |
RNAscope HiPlex12 CS Negative Control Probe |
ACD (Bio-Techne) 324347 |
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RNAscope HiPlex12 CS Positive Control Probe – Hs |
ACD (Bio-Techne) 324317 |
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RNAscope HiPlex12 CS Positive Control Probe – Mm |
ACD (Bio-Techne) 324437 |
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RNAscope HiPlex12 CS Positive Control Probe – Rn |
ACD (Bio-Techne) 324337 |
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Extras |
RNAscope Control Slide -Human Hela Cell Pellet |
ACD (Bio-Techne) 310045 |
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RNAscope HiPlex Cleaving Stock Solution v2 |
ACD (Bio-Techne) 324399 |
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Consumables |
COMET chip (20-pack) |
Lunaphore MK03 |
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Multistaining Buffer 20x |
Lunaphore BU06 |
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Dewax and HIER Buffer L (pH 6) |
Epredia AR02 |
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Dewax and HIER Buffer H, pH 9 |
Epredia AR03 |
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Imaging Buffer |
Lunaphore BU09 |
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Elution Buffer Kit |
Lunaphore BU07-L |
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Blocking Buffer |
Lunaphore BU10 |
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Quenching Buffer Kit |
Lunaphore BU08-L |
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20X SSC |
Thermo Fisher 15557044 |
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Xylene (or xylene-alternative, e.g. Histoclear) |
Merck H2779 |
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50 mL conical tubes |
Falcon 352070 |
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2 mL microtubes |
Eppendorf 0030123344 |
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SuperFrost Plus Adhesion Slides |
Epredia J1830AMNZ |
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Alexa Fluor™ Plus 488 Goat anti-Mouse IgG |
Thermo Fisher A32723 |
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Alexa Fluor™ Plus 488 Goat anti-Rabbit IgG |
Thermo Fisher A32731 |
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Alexa Fluor™ Plus 488 Goat anti-Rat IgG |
Thermo Fisher A48262 |
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Alexa Fluor™ Plus 555 Goat anti-Mouse IgG |
Lunaphore DR555MS |
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Alexa Fluor™ Plus 647 Goat anti-Mouse IgG |
Lunaphore DR647MS |
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Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG |
Lunaphore DR555RB |
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Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG |
Lunaphore DR555RB |
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Alexa Fluor™ Plus 555 Goat anti-Rat IgG |
Lunaphore DR555RT |
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Alexa Fluor™ Plus 647 Goat anti-Rat IgG |
Lunaphore DR647RT |
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Alexa Fluor™ 750 Goat anti-Rabbit IgG |
Thermo Fisher A-21039 |
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Alexa Fluor™ 750 Goat anti-Mouse IgG |
Thermo Fisher A-21037 |
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DAPI solution |
Lunaphore DR100 |
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SPYRE™ Immuno-Oncology Core Panel Kit, human |
This panel includes 13 validated monoclonal antibodies for FFPE human tissues: αSMA, CD3, CD4, CD8, CD11c, CD20, CD45, CD56, CD68, FOXP3, Ki-67, PD-1, PD-L1
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Lunaphore MP10010HU |