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Center for Microscopy and Image Analysis

CLSM - Zeiss LSM 900 (Schlieren)

ZEISS LSM 900

The Zeiss LSM 900 is an advanced inverted confocal laser scanning microscope designed for high-resolution fluorescence imaging. This confocal features two high-quality GaAsP detector, offering exceptional sensitivity and precision in image acquisition. The LSM 900 maintains a robust design suitable for a wide range of applications, from basic research to complex live cell studies, and is equipped with an environmental control box for temperature-sensitive experiments. This system represents a reliable and versatile solution for diverse imaging needs in scientific research.

Responsible Person

responsible person

Location

 University Zurich, Schlieren, Room WAD-12-K-105. 

Training Request

 Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope
  • Inverted confocal microscope (Zeiss LSM 900, Axio Observer 7)
  • Motorized x/y stage
  • Definite Focus 3
  • 3D capability
  • Time lapse capability
  • Water autoimmersion module
  • Incubator dark
  • CO2 and O2 module

Stage inserts
  • Universal mounting frame K-M
  • Insert for Universal mounting frame K-M, Petri dish 35
  • Insert for Universal mounting frame K-M, slides and chambers
  • Insert frame for 4 microscope slides 76 x 26 mm

Light Sources and Lasers
  • Solid-State Light Source Colibri 5
    • UV (385nm)
    • Blue (475nm)
    • Green (555nm)
    • Red (630nm)
  • Filter set 90 HE LED for use with the illumination system Colibri 5
    • Suitable for fluorescent dyes like DAPI, FITC, TRITC and Cy5
      • excitation wavelengths 385, 475, 555 and 630 nm
      • beam splitter QBS 405 + 493 + 575 + 653
      • emission filter QBP 425/30 + 514/30 + 592/25 + 709/100
  • Solide state diode lasers
    • 405nm, 5mW
    • 488nm, 10mW
    • 561nm, 10mW
    • 640nm, 5mW

Scanner
  • Confocal point-scanning - optical sectioning
  • Zoom function
  • Scan field rotation

Detector
  • 2x Gallium Arsenide Phosphid-PMT (GaAsP-PMT)
  • Main beam splitter (MBS) for 4 laser lines
    • 405, 488, 561 and 640nm

Available Optics
Name Magnification NA Immersion WD (mm)
ZEISS Objective Fluar 2.5x 0.12 Air 8.7
ZEISS Plan-Apochromat 10x 0.45 Air 2.1
ZEISS Plan-Apochromat 20x 0.8 Air 0.8
ZEISS LD LCI Plan-Apochromat 40x 1.2 water, silicon oil or glycerine 0.41
ZEISS Plan-Apochromat DIC 63x 1.4 Oil 0.19