CLSM - Evident Fluoview 4000 (Irchel)

The Evident Fluoview 4000 confocal laser scanning microscope is a high-end confocal imaging systems equipped with
- SilVIR Next-Generation Detector Technology (Silicon photomultiplier (SiPM).
- Extented near infrared (NIR) lasers will allow to expand your spectrar range from 405nm to 785 nm, making it possible to multiplex up to six channels simultaneously.
- Resonant scanner allowing high speed imaging.
- Stage top incubation system allows control of temperature and CO2 levels enabling live cell experiments.
Location
University Zurich, Irchel Campus, Room Y42-H-93.
Training Request
Follow this link to apply for an introduction to the microscope.
Technical Specifications
Microscope
- inverted microscope (Olympus Inverted IX83)
- motorized xyz stage (IX3-SSU)
- Olympus Z-drift compensator (IX3-ZDC2)
Light Sources and Lasers
- Epifluorescence Lightsource
- solid state LED and LDP - U-LGPS
- 360 to 665 nm
- Laser lines for confocal imaging:
- 405 nm, 445 nm, 488 nm, 561 nm, 594 nm, 640 nm
- NIR: 685 nm, 730 nm, 785 nm
Objectives
Name | Magnification | NA | Immersion | WD (mm) |
---|---|---|---|---|
PlanApo N | 2x | 0.08 | Air | 6.2 |
UCPLFLN20X | 20x | 0.7 | Air | 0.80 - 1.80 |
UPlanXApo | 40x | 0.95 | Air | 0.180 |
Silicon oil Immersion |
||||
UPLSAPO UPlan S Apo | 30x | 1.05 | Silicon oil | 0.8 |
UPLSAPO UPlan S Apo |
60x | 1.3 |
Silicon oil |
0.3 |
Fluorescence Filters for Epifluorescence
For further details please contact the responsible persons.
Detectors
Accessories
- Inserts for ibidi slides, well plates and 35mm dishes
- Incubation system for live cell imaging (cellVIVO)
- Allows for a variety of experimental protocols with 0 - 20 % CO2.
- Reliable control of temperature and CO2 with up to 0.05 °C accuracy
Literature and Links
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy