CLSM - Leica SP8 inverse (Schlieren)

The Leica SP8 is an automated inverted confocal laser scanning microscope allowing simultaneous acquisition of 4 fluorescent channels and 1 transmitted light channel (BF or DIC). A climate chamber and resonant scanner favor confocal live cell studies.

University Zurich, Schlieren, Room WAD-12-K-105. 

Follow this link to apply for an introduction to the microscope.

Microscope

• inverted confocal microscope (Leica DMI6000 AFC, Model SP8)
• 4 freely selectable emission detection windows
• motorized x/y stage
• 3D capability
• time lapse capability

Light Sources and Lasers

• Halogen lamp for transmitted light
• External fluorescence lamp  LQ-HXP 120
• Diode Lasers: 405 nm, 442nm
• Argon Laser: 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
• Diode pumped solid state laser (DPSS): 561nm
• Helium Neon (HeNe) Laser: 633nm

Scanners (tandem scanner system)

• confocal point-scanning - optical sectioning
• zoom function
• Regular scanner (field-of-view scanner): 1 Hz to 1800 Hz
• Resonant scanner: 8 kHz

Detectors

• 4 confocal fluorescence channels: two Photomultipliertubes (PMTs) and two super-sensitive Hybrid detectors (HyD)
• one transmission light-channel (PMT-Trans) 

Objectives

^{CS2 objectives: improved color correction, perfect VIS-405; IMM = multi immersion (either water, glycerol or oil)}

Fluorescence Filters

Accessories

• controlled environmental conditions (CO₂, temperature, humidity)
• Matrix screening software (reservation needed)

Literature and Links

Laser Scanning confocal Microscopy

ibiology - Optical Sectioning and Confocal Microscopy  

Leica Science Lab

Responsible Persons

If you have questions about the device please contact the responsible person.

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy