CLSM - Leica SP8 inverse (Schlieren)
The Leica SP8 is an automated inverted confocal laser scanning microscope allowing simultaneous acquisition of 4 fluorescent channels and 1 transmitted light channel (BF or DIC). A climate chamber and resonant scanner favor confocal live cell studies.
Location
University Zurich, Schlieren, Room WAD-12-K-105.
Training Request
Follow this link to apply for an introduction to the microscope.
Technical Specifications
Microscope
- inverted confocal microscope (Leica DMI6000 AFC, Model SP8)
- 4 freely selectable emission detection windows
- motorized x/y stage
- 3D capability
- time lapse capability
Light Sources and Lasers
- Halogen lamp for transmitted light
- External fluorescence lamp LQ-HXP 120
- Diode Lasers: 405 nm, 442nm
- Argon Laser: 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
- Diode pumped solid state laser (DPSS): 561nm
- Helium Neon (HeNe) Laser: 633nm
Scanners (tandem scanner system)
- confocal point-scanning - optical sectioning
- zoom function
- Regular scanner (field-of-view scanner): 1 Hz to 1800 Hz
- Resonant scanner: 8 kHz
Detectors
- 4 confocal fluorescence channels: two Photomultipliertubes (PMTs) and two super-sensitive Hybrid detectors (HyD)
- one transmission light-channel (PMT-Trans)
Objectives
| Name | Magnification | NA | Immersion | WD (mm) |
|---|---|---|---|---|
| HC PL FLUOTAR | 10x | 0.3 | Air | 11.0 |
| HC PL APO CS2 | 20x | 0.75 | IMM | 0.68 |
| PL APO CS | 20x | 0.7 | Air | N/A |
| HC PL APO CS2 | 63x | 1.4 | oil | 0.1 |
| HC PL APO CS2 | 63x | 1.3 | glycerol | 0.3 |
CS2 objectives: improved color correction, perfect VIS-405; IMM = multi immersion (either water, glycerol or oil)
Fluorescence Filters
| Name | Excitation Range | Excitation Filter | Dichroic | Emission Filter |
|---|---|---|---|---|
| A4 | UV | BP 360/40 | 400 | BP 470/40 |
| GFP | blue | BP 470/40 | 500 | BP 525/50 |
| N2.1 | green | BP 515-560 | 580 | LP 590 |
| CFP | violet/blue | BP 436/20 | 455 | BP 480/40 |
Accessories
- controlled environmental conditions (CO2, temperature, humidity)
- Matrix screening software (reservation needed)
Literature and Links
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy