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Center for Microscopy and Image Analysis

CLSM - Zeiss LSM 980 Airyscan (Irchel)

Zeiss_LSM980_Airy2_H79

The Zeiss LSM 980 Airyscan is an inverted confocal laser scanning microscope for spectral selective fluorescence imaging. The system is equipped with a sensitive 32 channel array GaAsP detector flanked by two conventional multialkali PMTs allowing for simultaneous acquisition of multicolor samples and spectral unmixing. The additional Airyscan 2 detector allows for fast and gentle super resolution and confocal image acquisition. For live cell studies, the microscope is equipped with an environmental control box.

Location

University Zurich, Irchel Campus, Room Y42-H-79. 

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope

  • Inverted confocal microscope (Zeiss LSM 980, Axio Observer 7)
  • Motorized x/y stage
  • 3D capability
  • Time lapse capability

Light Sources and Lasers

  • Halogen lamp for transmitted light
  • External fluorescence lamp HXP 120 V
  • Solide state diode lasers: 405nm, 445nm, 488nm, 514nm, 561nm, 639nm 

Scanner

  • Confocal point-scanning - optical sectioning
  • Zoom function
  • Scan field rotation
  • Airydetector with multiplex mode

Detectors

  • Spectral detector (GaAsP-PMT, 32 channels)
  • Two multialkali photonmultipliertubes (PMTs)
  • Airyscan 2 detector
  • Transmission light detector (T-PMT)

Airyscan 2 - Multiplex Mode

  • Airyscan module with super resolution (lateral resolution increase factor 1.7 for normal~, and up to a factor 1.5 for multiplex mode [SR-4Y, SR-8Y])
  • Airyscan Multiplex Mode can be also applied for fast confocal image acquisition (CO-8Y)
  • Airy Detector: concentrically-arranged hexagonal detector array (32 single detector elements, GaAsP-PMT area detector)
  • Airy Scan fast module beam shaper (illumination modulator fast airyscan for fast image acquisition)
Zeiss Airy detectors modes

Using a 63x, 1.4NA objective at 488nm laser excitation. Adapted from Zeiss.

Note: Filters for airy scan detection please find below (section Fluorescence Filters)

Objectives

Name Magnification NA Immersion WD (mm)
EC Plan-Neofluar 2.5x 0.085 Air 8.8
Plan-Apochromat 10x 0.45 Air 2.1
LD LCI Plan-Apochromat 25x 0.8 IMM1 0.57
LD LCI Plan-Apochromat 40x 1.2 IMM2 0.41
Plan-Apochromat 63x 1.4 Oil 0.19

IMM1 = multi immersion (either water, oil, glycerin or Silicon-oil); IMM2 = multi immersion (either water, glycerin or Silicon-oil)

Fluorescence Filters

Filter for Epi-Fluorescence using the filter turret

Camera

  • ZEISS Axiocam 506 mono
  • Pixel: 2752x 2208 (6 Megapixel)
    Pixel size: 4.54 μm x 4.54 μmFeff
    QE: 72% max. QE (@ 530 nm)
    Cooling: Regulated Peltier-cooling, sensor temperature 18 °C
    Bit depth: 14 bit
Name Excitation Range Excitation Filter Dichroic Emission Filter
DAPI UV G 365 FT 395 BP 445/50
GFP blue BP 470/40 FT 510 BP 515-565
Rhodamine green BP 546/12 FT 560 BP 575-640

Dual band Emission filters for Airy Scan Module

  • BP 420-480 + BP 495-550
  • BP 420-480 + BP 570-630
  • BP 420-500 + LP 605
  • BP 465-505 + BP 525-585
  • BP 495-550 + BP 570-630
  • BP 495-560 + LP 660
  • BP 570-620 + LP 655

Accessories

  • Environmental control box (CO2, temperature, humidity)
  • Three offline licenses for Airy scan processing on ZMB VMs

Literature and Links

LSM 9 family with Airy 2 - Technical Note (Zeiss)

 

Responsible Persons

If you have questions about the device please contact the responsible person.

 

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

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