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Center for Microscopy and Image Analysis

Widefield - Nikon Ti2-E (Irchel)

Nikon Eclipse Ti2

This inverted wildfield system is suited to image live cells as well as fixed samples in fluorescence and transmission light modes. A temperature controlled environment is possible for live cell experiments. The system does not have CO2 control. It is well suited for phase contrast imaging of bacteria and does allow complex imaging tasks.

Location

University Zurich, Irchel Campus, Room Y38-M-77.

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope

Nikon Eclipse Ti2-E fully motorized microscope:
- Motorized encoded xy-stage and motorized encoded z-drive
- Perfect Focus System 4
- Motorized fluorescence turret, filter-wheel, condenser turret, light path switcher

 

Light Sources

- Lumencor SpectraX with 6 individual LED-lines (395, 440, 470, 510, 550/575, and 640)

Name Magnification NA Immersion          WD (mm)
CFI Plan Apochromat Lambda    2x                            0.1 Air 8.5
CFI Plan Apochromat Lambda DIC  10x 0.45                Air 4.0
CFI Plan Apochromat Lambda PH2  20x 0.75 Air 1.0
CFI Plan Apochromat Lambda DIC  40x 0.95 Air 0.25
CFI Plan Apochromat Lambda PH2  40x 0.6 Air 3.6
CFI Plan Apochromat Lambda PH3 100x 1.45 Oil 0.13
          

Ph = phase contrast, DL = Dark Low Contrast, DM = Dark Medium Contrast, DIC = Differential Interference Contrast

triple filter cube CFP/YFP/mCherry Excitation Mirror Emission
  426-450 464-486 464-486
  497-519 532-554                532-554
  567-588 603-800 603-800
quadruple filter cube Dapi/FITC/TRITC/Cy5      
  352-404 414-450 414-450
  461-487 499-530 499-530
  543-566 580-611 580-611
  626-644 659-800 661-800

wavelength in nanometer

Emission filter wheel Emission
  414-450
  499-530
  580-611
  662-732

wavelength in nanometer

Camera Systems

Orca Fusion CoaXpress sCMOS

2304 x 2304 pixel (6.5 x 6.5um pixel size)

Accessories

  • IR Hardware Autofocus (Nikon Perfect Focus System - PFS)
  • Temperature and humidity control (Okolab)
  • NIS-Elements with JOBS module (enabling e.g. automatic screening of 96 or 384 well plates)

 

Responsible Persons

If you have questions about the device please contact the responsible person.

 

Make sure to acknowledge the Center for Microscopy and Image Analysis in your publication to support us.

How to acknowledge contributions of the Center for Microscopy and Image Analysis

 

Weiterführende Informationen

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