Sample Preparation Instruments

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E-beam coater - Bal-tec MED 020 (Irchel)

The Bal-tec coater MED 020 is a versatile high-vacuum coating device, primarily set-up for electron beam evaporation (carbon, carbon /platinum) and low-angle rotary shadowing. The system can also be used as sputtering device or for glow-discharging.
Sputter coater - Safematic CCU-010 (Irchel)

The Bal-tec sputter coater SCD500 is a high-vacuum sputter coating device.
Glow discharger - Balzers (Irchel)

The Balzers glow discharging device is exclusively used for glow discharging of TEM grids or other surfaces.
Critical point dryer - Bal-tec CPD 030 (Irchel)

The Bal-tec critical point dryer CPD030 is a standard critical point dryer to dry fixed and dehydrated biological samples for subsequent imaging in the scanning electron microscope.
Ultramicrotome - Leica Ultracut UCT (Irchel)

Ultramicrotome to produce semi-thin or ultra-thin sections of embedded or frozen hydrated samples for electron microscopy.
Ultramicrotome - Leica Artos 3D Box 3 (Irchel)

Ultramicrotome to produce semi-thin or ultra-thin sections of embedded or frozen hydrated samples for electron microscopy.
Freeze substitution - AFS2 Leica (Irchel)

Freeze-substitution device for dehydration (and embedding) of frozen-hydrated samples or for progressive lowering temperature dehydration and embedding.
High-pressure freezer - Leica EM HPM100 (Irchel)

High-pressure freezing for freezing of frozen hydrated samples without visible ice crystal damage.
Grid Plunger GP2 - Leica (Irchel)

Grid plunger to freeze samples for subsequent cryo-transmission electron microscopy
Freeze-fracturing system - Leica BAF060 (Irchel)

Freeze-fracturing device for fracturing of frozen hydrated samples for subsequent replica production or imaging in the cryo scanning electron microscope.
Cryo transfer system - Leica VCT100 (Irchel)

With the cryo transfer system VCT100, frozen-hydrated samples can be transferred contamination-free from LN2 to high-vacuum conditions, between high-vacuum systems (freeze-fracturing device and cryo scanning electron microscopy), and back to LN2 at atmospheric pressure.
Cryostat - CryoStar NX70 (Irchel)

The HYRAX C 60 is an electronic, optionally motorized cryostat with retraction that incorporates a unique refrigeration system for independent specimen and knife cooling. This facilitates applications in both the histology and research labs.
Vibratome - Leica VT1200 S (Irchel)

The HYRAX C 60 is an electronic, optionally motorized cryostat with retraction that incorporates a unique refrigeration system for independent specimen and knife cooling. This facilitates applications in both the histology and research labs.
Electrophoretic Tissue Clearing System

The X-CLARITYTM Electrophoretic Tissue Clearing System is used for rendering tissues and organs optically transparent in minimal time.
Flow bench - Scanlaf Safe 1200 Class 2 (Irchel)

Class 2 - biohazard safety cabinet with centrifuge, phase contrast microscope, with magnifications 10x, 20x, 40x and an incubator, 37°C, 5% CO2

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